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- Microscopy Basics
Calibration is only necesary if we wish to use the measurement, scale and grid functions of the software.
To calibrate the software with its Moticam or digital microscope, you will need to use the calibration slide supplied with said product.
We will select one of the four dots that appear on the calibration slide, using the appropriate dot based on the objective that we wish to calibrate.
Following the chart below, Motic recommends the following possible combinations of dot/objective to calibrate
|4X Objective||Dot 1500 μm.|
|10X Objective||Dot 600 μm.|
|40X Objective||Dot 150 μm.|
|100X Objective||Dot 70 μm.|
1- Once the dot is perfectly focused, and without it protruding from the field of view on the window, we will need to go to the "advanced settings" menu and select the option "calibration".
2- Select the "objective" that you are using to observe the dot on the calibration slide on the drop-down menu.
3- Select the "diameter" of the dot on the calibration slide on the drop-down menu.
4- After confirming the calibration, the selected area will automatically appear in green. In order to ensure the calibration has been completed successfully, the green area must match exactly with the area of the dot on the calibration slide.
5- If we see that the program has not detected the dot correctly, we will have to repeat the calibration by making the necessary adjustments to ensure the results are correct. For example:
6- You will then be able to overwrite and rename the objective related to the calibration to your liking. This will help you identify it later on on the calibration table. For example: 4X-Motic and "Save" the calibration.
7- It is posible to overlap the grid and scale functions to ensure the calibration has been carried out properly.
8- Once the calibration is finalized, this new objective will appear in the drop-down menu of the Motic Live Imaging Module, allowing you to select it.
9- With the Motic Images Plus 3.0 software, once we have calibrated, it is also possible to take measurements after the image has been captured. Go to "measurements", drop-down menu "objective"
Switch on the Microscope
Before switching on the microscope, plug-in the microscope to the power supply (1). Once this is done, switch it on (2) and gradually increase the intensity control (3).
Interpupillary distance adjustment
Before adjusting the interpupillary distance, place the sample on the specimen holder, and select the 10X objective (4).
Focus the sample by using the coarse focusing knob, which is located on the left side of the microscope’s stand (5).
Then, adjust the interpupillary distance so that both the right and left field of view become one image. This adjustment will allow the user to observe the specimen with both eyes (6).
The diopter adjustment compensates any differences in vision between the left and the right eye.
Look through the eyepiece on the right side with your right eye (7).
Focus the image by using the fine and/or coarse focusing adjustment knobs until the image of the specimen is sharp (8).
Then, look through the eyepiece on the left with your left eye. Use the diopter’s adjustment ring to focus the sample until the image of the specimen is sharp (9).
The microscope is now ready for perfect binocular viewing, according to the user’s dioptres, providing an accurate visualisation of the specimen.
Condenser adjustment (for microscopes with Kohler illumination)
Note: Other models without Koehler illuminator are supplied pre-centred.
To ensure optimal and homogeneous Koehler illumination, we need to make sure that the specimen and the Koehler diaphragm are both in focus. For this procedure, we recommend using the 10X objective.
First, focus the sample. Then, close the aperture of the diaphragm of the Koehler illumination (10).
Now, focus the diaphragm moving the condenser up or down using the condenser’s focusing knob (located under the mechanical stage on the left hand-side of the microscope) (11).
Once the diaphragm is focused at the same level of the specimen, centre the condenser by using the condenser’s centring screws, located on both sides of the condenser (12).
After all of this is done, open the aperture of the diaphragm. Now the illumination should be homogeneous on the whole field of view (13).
Condenser adjustment (of the iris diaphragm)
Note: When using the 100X objective, position the iris diaphragm aperture knob to the 0.9 value on the scale (or 100X engraved).
It is necessary to match the NA value of the objectives with the aperture of the iris diaphragm condenser.
Numerical Aperture is commonly used in microscopy to describe the angle of the cone of light that can enter or exit the objective lens; hence its light-gathering ability and resolution.
Align the aperture of the iris diaphragm ring matching it with the NA value on the scale (15). The scale value should match the NA number engraved on the objective (14), (some condensers show the position determined for each objective: 10X, 40X, etc...).
Using immersion oil objectives
Caution: Remove any air bubbles from the nozzle of the oil ampoule before use. Immersion oil must be used sparingly. After its use, the oil should be wiped off the 100X objective with a lens cleaning tissue, and the residual film should be removed with a solution made of ether (70%) and alcohol (30%).
Focus the sample with the 40X objective, locate your area of interest, and move the objective away from the light path (16).
Add one drop of immersion oil on the sample and move the 100X objective on the light path (17).
You will see that the oil will form a column-shaped drop between the sample and the objective’s lens (18).
Use the fine focusing knob to sharpen the image. Ensure no air bubbles have formed (19).
There is a wide network of distributors throughout Europe. In case you need a personal recommendation, please contact us through our website and we will forward your request to a sales partner close to your place.
The Motic Europe Limited Lifetime Warranty assures that the microscope is guaranteed against defects in material and workmanship for the life of the product. Normal wear, routine maintenance, light bulbs, batteries, fuses, damage resulting from repair by unauthorized parties, accident, alteration, shipping, misuse or abuse is not covered. Warranty service is provided by Motic Europe’s authorized technicians. The warranty period starts on the date of invoice. Determination of warranty is at the technician’s discretion.
Motic Europe has various categories of warranty:
- Electrical components are covered for 2 years
- For optical and mechanical components, depending on the product:
- 2-year Limited Warranty: Digital cameras (including built-in cameras within digital microscopes) and built-in tablets; as well as the MoticEasyScan Series.
- 5- year Limited Warranty: BA50-80 Series, EcoLine Series, BA Series (incl. MET), BA Elite Series, AE Series, POL Series, Panthera Series, SMZ Series, K Series, GM Series, PSM Series.
- 10- year Limited Warranty: Classic Line, Educational Line, RED Line, SWIFT Line.
- Lifetime Optical Warranty: SFC100 Series and ST30 Series.